Fluorescence based HTS-compatible ligand binding assays for dopamine D3 receptors in baculovirus preparations and live cells
Tahk M-J, Laasfeld T, Meriste E, Brea J, Loza MI, Majellaro M, Contino M, Sotelo E and Rinken A
Pharmacological insights emerging from the characterization of a large collection of synthetic cannabinoid receptor agonists designer drugs
Claudia Gioé-Gallo, Sandra Ortigueira, José Brea, Iu Raïch, Jhonny Azuaje, M Rita Paleo, Maria Majellaro, María Isabel Loza, Cristian O Salas, Xerardo García-Mera, Gemma Navarro, Eddy Sotelo
Multicomponent Reaction-Assisted Drug Discovery: A Time- and Cost-Effective Green Approach Speeding Up Identification and Optimization of Anticancer Drugs
Graziano, G.; Stefanachi, A.; Contino, M.; Prieto-Díaz, R.; Ligresti, A.; Kumar, P.; Scilimati, A.; Sotelo, E.; Leonetti, F.
Development of Fluorescent 4‐[4-(3H‐Spiro[isobenzofuran-1,4′- piperidin]-1′-yl)butyl]indolyl Derivatives as High-Affinity Probes to Enable the Study of σ Receptors via Fluorescence-Based Techniques
Francesca Serena Abatematteo, Maria Majellaro, Bianca Montsch, Rubén Prieto-Díaz, Mauro Niso, Marialessandra Contino, Angela Stefanachi, Chiara Riganti, Giuseppe Felice Mangiatordi, Pietro Delre, Petra Heffeter, Eddy Sotelo and Carmen Abate
Exploring the Effect of Halogenation in a Series of Potent and Selective A2B Adenosine Receptor Antagonists
Rubén Prieto-Díaz, Manuel González-Gómez, Hugo Fojo-Carballo, Jhonny Azuaje, Abdelaziz El Maatougui, Maria Majellaro, María I. Loza, José Brea, Víctor Fernández-Dueñas, M. Rita Paleo, Alejandro Díaz-Holguín, Beatriz Garcia-Pinel, Ana Mallo-Abreu, Juan C. Estévez, Antonio Andújar-Arias, Xerardo García-Mera, Iria Gomez-Tourino, Francisco Ciruela, Cristian O. Salas, Hugo Gutiérrez-de-Terán and Eddy Sotelo
N-adamantyl-anthranil amide derivatives: New selective ligands for the cannabinoid receptor subtype 2 (CB2R)
Giovanni Graziano, Pietro Delre, Francesca Carofiglio, Josè Brea, Alessia Ligresti, Magdalena Kostrzewa, Chiara Riganti, Claudia Gioè-Gallo, Maria Majellaro, Orazio Nicolotti, Nicola Antonio Colabufo, Carmen Abate, Maria Isabel Loza, Eddy Sotelo, Giuseppe Felice Mangiatordi, Marialessandra Contino, Angela Stefanachi, Francesco Leonetti
Development of N-(1-Adamantyl)benzamides as Novel Anti-Inflammatory Multitarget Agents Acting as Dual Modulators of the Cannabinoid CB2 Receptor and Fatty Acid Amide Hydrolase
Francesca Intranuovo, Leonardo Brunetti, Pietro DelRe, Giuseppe Felice Mangiatordi, Angela Stefanachi, Antonio Laghezza, Mauro Niso, Francesco Leonetti, Fulvio Loiodice, Alessia Ligresti, Magdalena Kostrzewa, Jose Brea, Maria Isabel Loza, Eddy Sotelo, Michele Saviano, Nicola Antonio Colabufo, Chiara Riganti, Carmen Abate, and Marialessandra Contino
Fluorescence based HTS- compatible ligand binding assays for dopamine D receptors in baculovirus preparations and live cells
Tahk M-J, Laasfeld T, Meriste E, Brea J, Loza MI, Majellaro M, Contino M, Sotelo E and Rinken A
Deciphering conformational selectivity in the A2A adenosine G protein-coupled receptor by free energy simulations
A2B adenosine receptor antagonists rescue lymphocyte activity in adenosine-producing patient-derived cancer models
Apple Hui Min Tay, Rubén Prieto-Díaz, Shiyong Neo, Le Tong, Xinsong Chen, Valentina Carannante, Björn Önfelt, Johan Hartman, Felix Haglund, Maria Majellaro, Jhonny Azuaje, Xerardo Garcia-Mera, Jose M Brea, Maria I Loza, Willem Jespers, Hugo Gutierrez-de-Teran, Eddy Sotelo, Andreas Lundqvist
J. Immunother Cancer, 2022, 10, e004592
https://jitc.bmj.com/content/10/5/e004592
Identification of A2BAR as a potential target in colorectal cancer using novel fluorescent GPCR ligands
Optimization of 2-Amino-4,6-diarylpyrimidine-5-carbonitriles as Potent and Selective A1 Antagonists
Cristina Val, Carlos Rodríguez-García, Rubén Prieto-Díaz, Abel Crespo, Jhonny Azuaje, Carlos Carbajales, Maria Majellaro, Alejandro Díaz-Holguín, José M. Brea, Maria Isabel Loza, Claudia Gioé-Gallo, Marialessandra Contino, Angela Stefanachi, Xerardo García-Mera, Juan C. Estévez, Hugo Gutiérrez-de-Terán and Eddy Sotelo
Exploring Non-orthosteric Interactions with a Series of Potent and Selective A3 Antagonists
Darío Miranda-Pastoriza, Rodrigo Bernárdez, Jhonny Azuaje, Rubén Prieto-Díaz, Maria Majellaro, Ashish V. Tamhankar, Lucien Koenekoop, Alejandro González, Claudia Gioé-Gallo, Ana Mallo-Abreu, José Brea*, M. Isabel Loza, Aitor García-Rey, Xerardo García-Mera, Hugo Gutiérrez-de-Terán*, and Eddy Sotelo*
Similarities and differences upon binditng of naturally occurring Δ9-tetrahydrocannabinol-derivatives to cannabinoid CB1 and CB2 receptors
RaichIu Raich, Rafael Rivas-Santisteban, Alejandro Lillo, Jaume Lillo, Irene Reyes-Resina, Xavier Nadal, Carlos Ferreiro-Vera, Verónica Sánchez de Medina, Maria Majellaro, Eddy Sotelo, Gemma Navarro, Rafael Franco
Identification of V6.51L as a selectivity hotspot in stereoselective A2B adenosine receptor antagonist recognition
Identification of V6.51L as a selectivity hotspot in stereoselective A2B adenosine receptor antagon…
Xuesong Wang, Willem Jespers, Rubén Prieto-Díaz, Maria Majellaro, Adriaan P IJzerman, Gerard JP van Westen, Eddy Sotelo, Laura H Heitman, Hugo Gutiérrez-de-Terán
Potent and Subtype-Selective Dopamine D2 Receptor Biased Partial Agonists Discovered via an Ugi-Based Approach
Ana Mallo-Abreu, Irene Reyes-Resina, Jhonny Azuaje, Rafael Franco, Aitor García-Rey, Maria Majellaro, Darío Miranda-Pastoriza, Xerardo García-Mera, Willem Jespers, Hugo Gutiérrez-de-Terán,
Gemma Navarro, and Eddy Sotelo*
Catalytic performance of a metal-free graphene oxide-Al2O3 composite assembled by 3D printing
JhonnyAzuaje, AdriánRama, AnaMallo-Abreu, Mónica G.Boado, MaríaMajellaro, Carmen R.Tubío, RubénPrieto, XerardoGarcía-Mera, FranciscoGuitián, EddySotelo, AlvaroGil
3,4-Dihydropyrimidin-2(1H)-ones as Antagonists of the Human A2B Adenosine Receptor: Optimization, Structure–Activity Relationship Studies, and Enantiospecific Recognition
María Majellaro, Willem Jespers, Abel Crespo, María J. Núñez, Silvia Novio, Jhonny Azuaje, Rubén Prieto-Díaz, Claudia Gioé, Belma Alispahic, José Brea*, María I. Loza, Manuel Freire-Garabal, Carlota Garcia-Santiago, Carlos Rodríguez-García, Xerardo García-Mera, Olga Caamaño, Christian Fernandez-Masaguer, Javier F. Sardina, Angela Stefanachi, Abdelaziz El Maatougui, Ana Mallo-Abreu, Johan Åqvist, Hugo Gutiérrez-de-Terán*, and Eddy Sotelo*
Nitrogen-Walk Approach to Explore Bioisosteric Replacements in a Series of Potent A2B Adenosine Receptor Antagonists
Ana Mallo-Abreu, Rubén Prieto-Díaz, Willem Jespers, Jhonny Azuaje, Maria Majellaro, Carmen Velando, Xerardo García-Mera, Olga Caamaño, José Brea, María I. Loza, Hugo Gutiérrez-de-Terán, and Eddy Sotelo
X‐Ray Crystallography and Free Energy Calculations Reveal the Binding Mechanism of A2A Adenosine Receptor Antagonists
Willem Jespers, Grégory Verdon, Jhonny Azuaje, Maria Majellaro, Henrik Keränen,† Xerardo García-Mera, Miles Congreve, Francesca Deflorian, Chris de Graaf, Andrei Zhukov, Andrew S. Doré, Jonathan S. Mason, Johan Åqvist,Robert M. Cooke, Eddy Sotelo and Hugo Gutiérrez-de-Terán
The European Research Network on Signal Transduction (ERNEST): Toward a Multidimensional Holistic Understanding of G Protein- Coupled Receptor Signaling
Martha E. Sommer, Jana Selent, Jens Carlsson, Chris De Graaf, David E. Gloriam, Gyorgy M. Keseru, Mickey Kosloff, Stefan Mordalski, Aurelien Rizk, Mette M. Rosenkilde, Eddy Sotelo, Johanna K. S. Tiemann, Andrew Tobin, Nina Vardjan, Maria Waldhoer, and Peter Kolb
ACS Pharmacol. Transl. Sci. 2020, 3, 2
Trifluorinated Pyrimidine-Based A2B Antagonists: Optimization and Evidence of Stereospecific Recognition
Ana Mallo-Abreu, María Majellaro, Willem Jespers, Jhonny Azuaje, Olga Caamaño, XerardoGarcía-Mera, José M.Brea, MaríaI.Loza, HugoGutieŕrez-de-Terań,
and Eddy Sotelo
Free-Energy Calculations for Bioisosteric Modifications of A3 Adenosine Receptor Antagonists
Zuzana Jandova, Willem Jespers, Eddy Sotelo, Hugo Gutiérrez-de-Terán and Chris Oostenbrink
Clinical validation of blood/brain glutamate grabbing in acute ischemic stroke.
Silva, A., Pérez-Díaz, A., Santamaría, M., Correa-Paz, C., Rodríguez-Yáñez, M., Ardá, A., Pérez-Mato, M., Iglesias-Rey, R., Brea, J., Azuaje, J., Sotelo, E., Sobrino, T., Loza, M., Castillo, J., Campos, F
Structural Mapping ogf Adenosine Receptor Mutations: Ligand Binding and Signaling Mechanisms.
Jespers, W., Schiedel, A. C., Heitman, L., Cooke, R., Leene, L., van Westen, G. J., Glorian, D. E., Muller, C. E., Sotelo, E., Gutiérrez de Terán, H.
Three-Dimensional Printing in Catalysis: Combining 3D Heterogeneous Copper and Palladium Catalysts for Multicatalytic Multicomponent Reactions
Díaz-Marta, A.S. Tubío, C.R., Carbajales, C., Fernandez, C., Escalante, L., Sotelo, E., Guitián, F., Barrio, V. L., Gil, A., Coelho, A.
Development of Fluorescent Probes that Target Serotonin 5HT2B receptors
Azuaje, J., López, Paula, Iglesias, A., De la Fuente, R., Pérez-Rubio, J. M., García, D., Pérez, D., Stepniewski, T., García-Mera, X., Loza, M. I., Cadavid, M. I. Brea, J., Sotelo, E.*
Effect of Nitrogen Atom Substitution in A3 Adenosine Receptor Binding: N(4,6-Dirarylpyridin-2-yl)acetamides as Potent and Selective Antagonists
Azuaje, J., Jespers, W., Yaziji, V., Mallo, A., Majellaro, M., Caamaño, O., Loza, M. I., Cadavid, M. I. Brea, J. Gutiérrez de Terán, H., Sotelo, E.*
3-Oxopyridazin-5-yl-Chalcone Hybrids: Potent Antiplatelet Agents that Prevent GP IIb/IIIa Activation
El Maatougui, A., Yañez, M., Crespo, A., Fraiz, N., Coelho, A., Raviña, E., Cano, E., Loza, M. I., Brea, J., Gutiérrez de Terán, H., Sotelo, E.*
Synthesis and Characterization of a New Bivalent Ligand Combining Caffeine and Docosahexaenoic Acid
Fernandez-Dueñas, V., Morato, X., Ciruela, F., Azuaje, J., Sotelo, E.* Cordobilla, B., Domingo, J.C.
Enantiospecific Recognition at the A2B Adenosine Receptor by Alkyl 2-Cyanoimino-4-substituted-6-methyl-1,2,3,4-tetrahydropyrimiidine-5-carboxylates
Carbajales, C., Azuaje, J., Oliveira, A., Loza, M.I., Brea, J., Cadavid, M.I., Masaguer, C. F., García-Mera, X., Gutiérrez de Terán, H., Sotelo, E.*
An Efficient and Recyclable 3D Printed α-Al2O3 Catalyst for the Multicomponent Assembly of Bioactive Heterocycles
Azuaje, J., Tubio, C.R., Escalante, L., Gomez, M., Coelho, A., Caamaño, O., Gil, A., Sotelo, E.*
Multicomponent Assembly of the Kinesin Spindle Protein Inhibitor CPUYJ039 and Analogues as Antimitotic Agents.
Carbajales, C., Sawada, J-C., Marzano, G., Sotelo, E., Escalante, L., Sanchez-Diaz Marta, A., García-Mera, X., Asai, A., Coelho, A.
Structure-Based Rational Design of Adenosine Receptor Ligands.
Gutiérrez-de-Terán, H. Sallander, J. Sotelo, E.
Drug Structural Features Affect Drug Delivery from Hyperbranched Polyesteramide Hot Melt Extrudates.
Raviña-Eirín, E., Azuaje, J., Sotelo, E. Gómez-Amoza, J. L., Martínez-Pacheco, R.
Discovery of Potent and Highly Selective Adenosine A2B Receptor Antagonists Chemotypes.
El Maatougi, A., Azuaje, J., González-Gómez, M., Míguez, G., Crespo, A., García-Mera, X., Gutiérrez de Terán, H., Sotelo, E.*
3D-Printing of a Heterogeneous Copper-Based Catalyst
Tubio, C. R., Azuaje, J., Escalante, L., Coelho, A., Sotelo, E. Gil, A.
Pyrazin-2(1H)-ones as a Novel Class of Selective A3 Adenosine Receptor Antagonists.
Azuaje, J., Carbajales, C, González-Gomes, M., Coelho, A., Caamaño, O., Gutierrez de Terán, H., Sotelo, E.
Sol-Gel Entrapped Cu in Silica Matrix: An Efficienct Heterogeneous Nanocatalyst For Huisgen, and Ullmann Reactions.
Diz, P., Gil, A., Guitían, F., El Maatougui, A., Azuaje, J., Pernas, P., Sotelo, E., Coelho, A.
Integrated Ugi-Based Assembly of Functional, Skeletal and Stereochemically Diverse 1,4-Benzodiazepin-2-ones.
Azuaje, J., Pérez-Rubio, J. M., Yaziji, V., El Maatougui, González-Gómez, J. C., Masaguer, C. F., Sánchez-Pedregal, V., Navarro-Vázquez, A., Teijeira, M., Sotelo, E.*
Computer-Aided Design of GPCR Ligands.
Gutiérrez-de-Terán, H., Keranen, H., Azuaje, J., Rodriguez, D., Sotelo, E.
Structure-Based Design of Novel KSP-Eg5 Inhibitors Assisted by a Targeted Multicomponent Reaction.
Carbajales, C., Prado, M. A., Gutiérrez-de-Terán, H., Cores, A., Azuaje, J., Novio, S., Nuñez, M. J., Fernández, B., Sotelo, E., García-Mera, X., Sánchez-Lazo, P., Freire-Garabal, M., Coelho, A.
Ugi-Based Approaches to Quinoxaline Libraries.
Azuaje, J., El Maatougui, García-Mera, X., Sotelo, E.*
Discovery of 3,4-Dihydropyrimidin-2(1H)-ones as a Novel Class of Potent and Selective A2B Adenosine Receptor Antagonists.
Crespo, A., El Maatougui, A.,Biagini, P., Azuaje, J., Coelho, A., García-Mera, X., Brea, J., Loza, M. I., Cadavid, M. I., Sotelo, E.*
Copper-Catalyzed Huisgen 1,3-Dipolar Cycloaddition Under Oxidative Conditions: Polymer-Assisted Assembly of 4-Acyl-1-Substituted-1,2,3-Triazoles
Diz, P., Coelho, A., El Maatougui, A., Azuaje, J., Caamaño, O., Gil, A., Sotelo, E.*
Three-Component Assembly of Structurally Diverse 2-Aminopyrimidine-5-carbonitriles.
Val, C., Crespo, A., El Maatougui, A., Azuaje, J. A., Coelho, A., Sotelo, E.*
Multicomponent Assembly of Diverse Pyrazin-2(1H)-one Chemotypes
Azuaje, J., El Maatougui, A., Pérez-Rubio, J. M., Coelho, A., Fernández, F. Sotelo, E.*
Selective and potent Adenosine A3 Receptor Antagonists by Methoxyaryl Substitution on the N-(2,6-Diarylpyrimidin-4-yl)acetamide scaffold.
Yaziji, V., Rodríguez, D., Gutiérrez de Terán, H., Coelho, A., El Maatougui, A., Brea, J., Loza, M. I., Garcia-Mera, X., Cadavid, M.I., Sotelo, E.*
Discovery and Preliminary SAR of 5-Arylidene-2,2,-dimethyl-1,3-dioxane-2,4-diones as Platelet Aggregation Inhibitors.
El Maatougui, A., Azuaje, J., Coelho, A., Cano, E., Yañez, M., Yaziji, V., Carbajales, C., Sotelo, E.*
Pyrimidine Derivatives as Potent and Selective A3 Adenosine Receptor Antagonists.
. Yaziji, V., Rodríguez, D., Gutiérrez de Terán, H., Coelho, A., Brea, J., Loza, M. I., Garcia-Mera, X., Cadavid, M.I., Caamaño, Sotelo, E.*
Supported Tosic Acid as Highly Robust and Eco-Friendly Isocyanide´s Scavengers.
Azuaje, J., Al Maatougui, A., Blanco, J.M., Coelho, A., Sotelo, E.*
Silica-Supported Aluminum Chloride-Assisted Solution Phase Synthesis of Pyridazinone-Based Antiplatelet Agents.
El Maatougui, A., Azuaje, J., Sotelo, E., Caamaño, O., Coelho, A.
Convergent Assembly of Structurally Diverse Quinazolines.
Crespo, A., Coelho, A., Diz, P., Fernández, F., Novoa de Armas, H., Sotelo, E.*
Polymer Supported 1,5,7-triazabicylo[4,4,0]dec-5-ene as Polyvalent Ligands in the Copper Catalysed Huisgen 1,3-Dipolar Cycloaddition.
Coelho, A., Díz, P., Caamaño, O., Sotelo, E.*
Divergent Solution Phase Synthesis of Diarylpirimidines as Selective A3 Adenosine Receptor Antagonsits
Yaziji, V., Coelho, A., El Maatougui, Brea, J., Loza, M. I., Garcia-Mera, X., Sotelo, E.*
Synthetic Applications of Polystyrene 1,1,3,3-Tetramethylguanidine
Coelho, A., Crespo, A., Fernández, F., Biagini, P., Stefanachi, A., Sotelo, E.*
Click Chemistry Approach to Assembly Proline Mimetic Libraries Containing 1,4-Substituted 1,2,3-Triazoles.
Rodriguez-Borges, J. E., Gonçalves, S., do Vale, M. L., García-Mera, X., Coelho, A., Sotelo, E.
Design, Synthesis and Biological Evaluation of Glycine-Based Molecular Tongs as Inhibitors of α-β 1-40 Aggregation in Vitro.
Cellamare, S., Stefanachi, A., Antonella, S., Basile, T., Catto, M., Campagna, F., Sotelo, E., Carotti, A.
1,3- and 8-Substituted-9-Deazaxanthines as Potent and Selective Antagonists at the Human A2B Adenosine Receptor
Stefanachi, A., Brea, J. M., Cadavid M. I., Centeno, N. B., Esteve, C., Loza, M. I., Martínez, A., Nieto, R., Raviña, E., Sanz, F., Segarra, V., Sotelo, E., Vidal, B., Carotti, A.
2-Substituted 4-, 5- and 6-[(1E)-3-oxo-3-phenylprop-1-en-1-yl]pyridazin-3(2H)-ones and 2-Substituted 4,5-bis[(1E)-3-oxo-3-phenylprop-1-en-1-yl]pyridazin-3(2H)-ones as Potent Platelet Aggregation Inhibitors: Design, Synthesis and SAR Studies.
Meyers, C., Yáñez, M., Elmatougi, A., Verhest, T., Coelho, A., Fraiz, N., Lemière, G. L. F., García-Mera, X., Laguna, R., Cano, E., Maes, B. U. W., Sotelo, E.*
Synthesis, Adenosine Receptor Binding and 3D-QSAR- of 4-Substituted 2-(2´-furyl)-1,2,4-triazolo[1,5-a]quinoxalines.
Martínez A., Gutierrez de Terán, H., Brea, J. M., Raviña, E., Loza, M. I., Cadavid, M. I., Sanz, F., Segarra, V., Vidal, B. Sotelo, E.*
Design, Synthesis and Structure Activity Relationships of a Novel Series of 5-Alkylidenenpyridazin-3-ones with a non c-AMP-Based Antiplatelet Activity.
Coelho, A., Raviña, E., Yáñez, M., Fraiz, N., Laguna, R., Cano, E., Sotelo, E.*
Parallel Regulation By Olanzapine Of The Patterns Of Expression Of 5-Ht2a And D3 Receptors In Rat Central Nervous System And Blood Cells.
Padín; J. F., Rodríguez; M.A., Domínguez; E., Dopeso-Reyes; I. G., Buceta; M., Cano; E., Sotelo, E., Caruncho; H. J., Cadavid; M. I., Castro; M., Loza, M.I.
Expanding the Chemical Diversity of Azinone Libraries by A Consecutive Alkylation/Palladium-Catalysed Functionalization Strategy
Reactivity of 5-Alkynylpyridazinones Toward Hydrochloric Acid
Coelho, A., Novoa, H., Peeters, O.M., Blaton, N., Sotelo, E.*
Reactivity of 5-Iodopyridazin-3(2H)-ones in Palladium-Catalysed Reactions
Coelho, A., Sotelo, E., Novoa, H., Peeters, O.M., Blaton, N., Raviña, E.
8-Substituted-9-deazaxanthines as Adenosine Receptor Ligands: Design, Synthesis and Structure-affinity Relationships at A2B
Carotti, A., Stefanachi, A., Raviña, E., Sotelo, E., Loza, M. I., Cadavid, M. I., Centeno, N. B.
Efficient Heck Alkenylation at position 5 of the 6-phenyl-3(2H)-pyridazinone System.
Coelho, A., Sotelo, E., Novoa, H., Blaton, N., Peeters, O.M., Raviña, E.
Facile Solution Phase Combinatorial Synthesis of Highly Substituted Pyridazinones
Stille-Based Approaches in the Synthesis of 6-phenyl-5-Substituted-3(2H)-pyridazinones.
Sotelo, E., Coelho, A., Raviña, E.
Sonogashira Approaches in The Synthesis of 5-Substituted-6-phenyl-3(2H)-pyridazinones.
Coelho, A., Sotelo, E., Raviña, E.
Retro-Ene-Assisted Palladium-Catalysed Synthesis of 4,5-Disubstituted-3(2H)-pyridazinones.
Sotelo, E., Coelho, A., Raviña, E.
Traceless Solid Phase Synthesis of 4,5- and 5,6-Diaryl-3(2H)-pyridazinones.
